From: James Kozloski <optipro_optonline.com>
To: yehouda harpaz <yh@maldoo.com>; <rafa_cub.bio.columbia.edu>
Sent: Tuesday, August 14, 2001 9:12 PM
Subject: Re: Stereotypic position of synaptic targets

Dear Yehouda,

These experiments were done in coronal slices that were 350 um thick.  Fura
staining penetrates to around 3 cell layers into the slice (i.e., around 75
um).  Therefore, because our sampling method depended on Fura staining, we
were only able to probe 75 um along the axis perpendicular to the plane of
the slice.  This is why all the spatial analysis was done in just 2
dimensions.

Within these two dimensions, we expected to find high variance in the
distribution of followers, but did not.  Please note also that the two
dimensions analyzed included one axis perpendicular to cortex, (A<=>B in
Fig. 2), and one axis within the plane of the cortex (M<=>L in Fig. 2).

Best Regards,

James Kozloski



yehouda harpaz wrote:

> Dear James Kozloski,
>
> I am writing to you about your recent paper "Stereotyped position of
> local synaptic targets in neocortex". As far I can tell, all the spatial
> analysis in this paper is in a plane perpendicular to the plane of
> the cortex. Presumably, you also have the data about the relative
> position of the "followers" with respect to the triggers in the plane
> of the cortex. Why didn't you present it too? Are you going to
> publish it? Can you make it available electronically?
>
> Thanks,
>
> Yehouda Harpaz

From: James R Kozloski <kozloski_us.ibm.com>
To: yehouda harpaz <yh@maldoo.com>
Sent: Tuesday, August 14, 2001 10:52 PM
Subject: Re: Stereotypic position of synaptic targets



Ref. 26 describes the optical probing method.
Ref. 27 describes our slicing protocal.

Also,  figures 2a- c and their legends should make clear that we were
observing the surface of a slice of cortex, and not imaging the cortex in
3-D.

I hope this helps.  Science's strict page restrictions inevitably lead to
this kind of confusion.  My regrets.

Best regards,

James Kozloski


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